Journal: Investigative Ophthalmology & Visual Science
Article Title: EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development
doi: 10.1167/iovs.62.2.31
Figure Lengend Snippet: Deletion of EMC3 activates the ERS response and apoptosis. ( A ) E15.5, E17.5, and P0.5 retinal cryostat sections were immunostained with an antibody for pIRE1α ( red ). White arrows indicate the cells with activated IRE1α. ( B ) Western blot and quantitation were performed to detect ATF6 (50 kDa). ( C ) TUNEL assay ( green ) indicated apoptosis, which appeared in CKO mouse retinas beginning at E15.5. White circles show that apoptotic cells appeared in the center of the rosettes. Scale bars: 50 µm.
Article Snippet: EMC3 antibody (mouse, 1:500; HPA042372; Sigma-Aldrich), cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6) antibody (rabbit, 1:500; NBP1-40256; Novus Biologicals), and α-tubulin antibody (mouse, 1:1000; 660331-1-lg; ProteinTech Group, Rosemont, IL, USA) were incubated overnight at 4°C.
Techniques: Western Blot, Quantitation Assay, TUNEL Assay